The flow rate of 1000 ul/hr is the maximum flow rate that is achievable in our current instrumentation. It provided a fluid shear force of 0.3 dyne/cm2, which is a the low end of the physiologic shear forces seen in the post-capillary venule of the intestinal microvasculature , where most immune cell traffic and the normal shear rates are ~0.5 – 2 dyne/cm2.