Home Forums *Perspectives How do you validate 3D in vitro culture systems?  Reply To: How do you validate 3D in vitro culture systems? 

Stephen Riffle

@Chris — That makes a lot of sense. Is there any benchmark for determining what minimum criteria the model should meet? For example, if the Gut-Chip shows signature cytokine and permeability responses, but is lacking a cell-surface biomarker, would the model be invalidated or just caveated? Not sure if that example makes complete sense, but I’m more diving in on how much similarity with in vivo conditions is enough, and how much dissimilarity is too much?

My guess is that there’s no hard cut off. But do you think there’s the potential to work towards an IBD checklist/grading scale, so to speak, to allow for the same minimal validation criteria to be met across laboratories?